diff --git a/src/util/PathUtil.ts b/src/util/PathUtil.ts
index aa2f8a830..46541fa8f 100644
--- a/src/util/PathUtil.ts
+++ b/src/util/PathUtil.ts
@@ -5,6 +5,45 @@ import type { ResourceIdentifier } from '../http/representation/ResourceIdentifi
 import type { HttpRequest } from '../server/HttpRequest';
 import { BadRequestHttpError } from './errors/BadRequestHttpError';
 
+// Characters to ignore when URL decoding the URI path to a file path.
+const pathComponentDelimiters = [ '/', '\\' ];
+// Regex to find all instances of encoded path component delimiters.
+const encodedDelimiterRegex = new RegExp(
+  `${pathComponentDelimiters.map((delimiter): string => encodeURIComponent(delimiter)).join('|')}`, 'giu',
+);
+// Mapping of the replacements to perform in the preventDelimiterDecoding helper function.
+const preventDelimiterDecodingMap = Object.fromEntries(pathComponentDelimiters.map((delimiter): [string, string] => {
+  const encodedDelimiter = encodeURIComponent(delimiter);
+  return [ encodedDelimiter, encodeURIComponent(encodedDelimiter) ];
+}));
+// Mapping of the replacements to perform in the preventDelimiterEncoding helper function.
+const preventDelimiterEncodingMap = Object.fromEntries(pathComponentDelimiters.map((delimiter): [string, string] => {
+  const encodedDelimiter = encodeURIComponent(delimiter);
+  return [ encodedDelimiter, delimiter ];
+}));
+
+/**
+ * Prevents some characters from being URL decoded by escaping them.
+ * The characters to 'escape' are declared in codecExceptions.
+ *
+ * @param pathComponent - The path component to apply the escaping on.
+ * @returns A copy of the input path that is safe to apply URL decoding on.
+ */
+function preventDelimiterDecoding(pathComponent: string): string {
+  return pathComponent.replace(encodedDelimiterRegex, (delimiter): string => preventDelimiterDecodingMap[delimiter]);
+}
+
+/**
+ * Prevents some characters from being URL encoded by escaping them.
+ * The characters to 'escape' are declared in codecExceptions.
+ *
+ * @param pathComponent - The path component to apply the escaping on.
+ * @returns A copy of the input path that is safe to apply URL encoding on.
+ */
+function preventDelimiterEncoding(pathComponent: string): string {
+  return pathComponent.replace(encodedDelimiterRegex, (delimiter): string => preventDelimiterEncodingMap[delimiter]);
+}
+
 /**
  * Changes a potential Windows path into a POSIX path.
  *
@@ -118,6 +157,11 @@ function transformPathComponents(path: string, transform: (part: string) => stri
 /**
  * Converts a URI path to the canonical version by splitting on slashes,
  * decoding any percent-based encodings, and then encoding any special characters.
+ * This function is used to clean unwanted characters in the components of
+ * the provided path.
+ *
+ * @param path - The path to convert to its canonical URI path form.
+ * @returns The canonical URI path form of the provided path.
  */
 export function toCanonicalUriPath(path: string): string {
   return transformPathComponents(path, (part): string =>
@@ -125,17 +169,29 @@ export function toCanonicalUriPath(path: string): string {
 }
 
 /**
- * Decodes all components of a URI path.
+ * This function is used when converting a URI to a file path. Decodes all components of a URI path,
+ * with the exception of encoded slash characters, as this would lead to unexpected file locations
+ * being targeted (resulting in erroneous behaviour of the file based backend).
+ *
+ * @param path - The path to decode the URI path components of.
+ * @returns A decoded copy of the provided URI path (ignoring encoded slash characters).
  */
 export function decodeUriPathComponents(path: string): string {
-  return transformPathComponents(path, decodeURIComponent);
+  return transformPathComponents(path, (part): string =>
+    decodeURIComponent(preventDelimiterDecoding(part)));
 }
 
 /**
- * Encodes all (non-slash) special characters in a URI path.
+ * This function is used in the process of converting a file path to a URI. Encodes all (non-slash)
+ * special characters in a URI path, with the exception of encoded slash characters, as this would
+ * lead to unnecessary double encoding, resulting in a URI that differs from the expected result.
+ *
+ * @param path - The path to encode the URI path components of.
+ * @returns An encoded copy of the provided URI path (ignoring encoded slash characters).
  */
 export function encodeUriPathComponents(path: string): string {
-  return transformPathComponents(path, encodeURIComponent);
+  return transformPathComponents(path, (part): string =>
+    encodeURIComponent(preventDelimiterEncoding(part)));
 }
 
 /**
diff --git a/test/integration/FileBackendEncodedSlashHandling.test.ts b/test/integration/FileBackendEncodedSlashHandling.test.ts
new file mode 100644
index 000000000..a4e6293ca
--- /dev/null
+++ b/test/integration/FileBackendEncodedSlashHandling.test.ts
@@ -0,0 +1,149 @@
+import fetch from 'cross-fetch';
+import { pathExists } from 'fs-extra';
+import type { App } from '../../src/init/App';
+import { getPort } from '../util/Util';
+import {
+  getDefaultVariables,
+  getTestConfigPath,
+  getTestFolder,
+  instantiateFromConfig,
+  removeFolder,
+} from './Config';
+
+const port = getPort('FileBackendEncodedSlashHandling');
+const baseUrl = `http://localhost:${port}/`;
+
+const rootFilePath = getTestFolder('file-backend-encoded-slash-handling');
+
+describe('A server with a file backend storage', (): void => {
+  let app: App;
+
+  beforeAll(async(): Promise<void> => {
+    await removeFolder(rootFilePath);
+    const variables = {
+      ...getDefaultVariables(port, baseUrl),
+      'urn:solid-server:default:variable:rootFilePath': rootFilePath,
+    };
+
+    // Create and start the server
+    const instances = await instantiateFromConfig(
+      'urn:solid-server:test:Instances',
+      [
+        getTestConfigPath('server-file.json'),
+      ],
+      variables,
+    ) as Record<string, any>;
+    ({ app } = instances);
+
+    await app.start();
+  });
+
+  afterAll(async(): Promise<void> => {
+    // Await removeFolder(rootFilePath);
+    await app.stop();
+  });
+
+  it('can put a document for which the URI path contains url encoded separator characters.', async(): Promise<void> => {
+    const url = `${baseUrl}/c1/c2/t1%2F`;
+    const res = await fetch(url, {
+      method: 'PUT',
+      headers: {
+        'content-type': 'text/plain',
+      },
+      body: 'abc',
+    });
+    expect(res.status).toBe(201);
+    expect(res.headers.get('location')).toBe(url);
+
+    // The resource should not be accessible through ${baseUrl}/c1/c2/t1/.
+    const check1 = await fetch(`${baseUrl}/c1/c2/t1/}`, {
+      method: 'GET',
+      headers: {
+        accept: 'text/plain',
+      },
+    });
+    expect(check1.status).toBe(404);
+
+    // Check that the created resource is not a container
+    const check2 = await fetch(url, {
+      method: 'GET',
+      headers: {
+        accept: 'text/plain',
+      },
+    });
+    const linkHeaderValues = check2.headers.get('link')!.split(',').map((item): string => item.trim());
+    expect(linkHeaderValues).not.toContain('<http://www.w3.org/ns/ldp#Container>; rel="type"');
+
+    // Check that the appropriate file path exists
+    const check3 = await pathExists(`${rootFilePath}/c1/c2/t1%2F$.txt`);
+    expect(check3).toBe(true);
+  });
+
+  it('can post a document using a slug that contains url encoded separator characters.', async(): Promise<void> => {
+    const slug = 't1%2Faa';
+    const res = await fetch(baseUrl, {
+      method: 'POST',
+      headers: {
+        'content-type': 'text/plain',
+        slug,
+      },
+      body: 'abc',
+    });
+    expect(res.status).toBe(201);
+    expect(res.headers.get('location')).toBe(`${baseUrl}${slug}`);
+
+    // Check that the the appropriate file path exists
+    const check = await pathExists(`${rootFilePath}/${slug}$.txt`);
+    expect(check).toBe(true);
+  });
+
+  it('prevents accessing a document via a different identifier that results in the same path after url decoding.',
+    async(): Promise<void> => {
+      // First put a resource using a path without encoded separator characters: foo/bar
+      const url = `${baseUrl}/foo/bar`;
+      await fetch(url, {
+        method: 'PUT',
+        headers: {
+          'content-type': 'text/plain',
+        },
+        body: 'abc',
+      });
+
+      // The resource at foo/bar should not be accessible using the url encoded variant of this path: foo%2Fbar
+      const check1 = await fetch(`${baseUrl}/foo%2Fbar`, {
+        method: 'GET',
+        headers: {
+          accept: 'text/plain',
+        },
+      });
+      // Expect foo%2Fbar to correctly refer to a different document, which does not exist.
+      expect(check1.status).toBe(404);
+
+      // Check that the the appropriate file path for foo/bar exists
+      const check2 = await pathExists(`${rootFilePath}/foo/bar$.txt`);
+      expect(check2).toBe(true);
+
+      // Next, put a resource using a path with an encoded separator character: bar%2Ffoo
+      await fetch(`${baseUrl}/bar%2Ffoo`, {
+        method: 'PUT',
+        headers: {
+          'content-type': 'text/plain',
+        },
+        body: 'abc',
+      });
+
+      // The resource at bar%2Ffoo should not be accessible through bar/foo
+      const check3 = await fetch(`${baseUrl}/bar/foo`, {
+        method: 'GET',
+        headers: {
+          accept: 'text/plain',
+        },
+      });
+      // Expect bar/foo to correctly refer to a different document, which does not exist.
+      expect(check3.status).toBe(404);
+
+      // Check that the the appropriate file path for bar%foo exists
+      const check4 = await pathExists(`${rootFilePath}/bar%2Ffoo$.txt`);
+      expect(check4).toBe(true);
+    });
+});
diff --git a/test/integration/config/server-file.json b/test/integration/config/server-file.json
new file mode 100644
index 000000000..9b5fe98aa
--- /dev/null
+++ b/test/integration/config/server-file.json
@@ -0,0 +1,54 @@
+{
+  "@context": "https://linkedsoftwaredependencies.org/bundles/npm/@solid/community-server/^4.0.0/components/context.jsonld",
+  "import": [
+    "files-scs:config/app/main/default.json",
+    "files-scs:config/app/init/initialize-root.json",
+    "files-scs:config/app/setup/disabled.json",
+    "files-scs:config/http/handler/default.json",
+    "files-scs:config/http/middleware/websockets.json",
+    "files-scs:config/http/server-factory/websockets.json",
+    "files-scs:config/http/static/default.json",
+    "files-scs:config/identity/access/public.json",
+    "files-scs:config/identity/handler/default.json",
+    "files-scs:config/identity/ownership/token.json",
+    "files-scs:config/identity/pod/static.json",
+    "files-scs:config/identity/registration/enabled.json",
+    "files-scs:config/ldp/authentication/dpop-bearer.json",
+    "files-scs:config/ldp/authorization/webacl.json",
+    "files-scs:config/ldp/handler/default.json",
+    "files-scs:config/ldp/metadata-parser/default.json",
+    "files-scs:config/ldp/metadata-writer/default.json",
+    "files-scs:config/ldp/modes/default.json",
+    "files-scs:config/storage/backend/file.json",
+    "files-scs:config/storage/key-value/resource-store.json",
+    "files-scs:config/storage/middleware/default.json",
+    "files-scs:config/util/auxiliary/acl.json",
+    "files-scs:config/util/identifiers/suffix.json",
+    "files-scs:config/util/index/default.json",
+    "files-scs:config/util/logging/winston.json",
+    "files-scs:config/util/representation-conversion/default.json",
+    "files-scs:config/util/resource-locker/memory.json",
+    "files-scs:config/util/variables/default.json"
+  ],
+  "@graph": [
+    {
+      "@id": "urn:solid-server:test:Instances",
+      "@type": "RecordObject",
+      "RecordObject:_record": [
+        {
+          "RecordObject:_record_key": "app",
+          "RecordObject:_record_value": { "@id": "urn:solid-server:default:App" }
+        }
+      ]
+    },
+    {
+      "@id": "urn:solid-server:default:EmailSender",
+      "@type": "BaseEmailSender",
+      "args_senderName": "Solid Server",
+      "args_emailConfig_host": "smtp.example.email",
+      "args_emailConfig_port": 587,
+      "args_emailConfig_auth_user": "alice@example.email",
+      "args_emailConfig_auth_pass": "NYEaCsqV7aVStRCbmC"
+    }
+  ]
+}
diff --git a/test/unit/util/PathUtil.test.ts b/test/unit/util/PathUtil.test.ts
index 0d5c49ee4..2ae158e58 100644
--- a/test/unit/util/PathUtil.test.ts
+++ b/test/unit/util/PathUtil.test.ts
@@ -112,6 +112,11 @@ describe('PathUtil', (): void => {
     it('leaves the query string untouched.', (): void => {
       expect(decodeUriPathComponents('/a%20path&/name?abc=def&xyz')).toBe('/a path&/name?abc=def&xyz');
     });
+
+    it('ignores url encoded path separator characters.', (): void => {
+      expect(decodeUriPathComponents('/a%20path&/c1/c2/t1%2F')).toBe('/a path&/c1/c2/t1%2F');
+      expect(decodeUriPathComponents('/a%20path&/c1/c2/t1%5C')).toBe('/a path&/c1/c2/t1%5C');
+    });
   });
 
   describe('#encodeUriPathComponents', (): void => {
@@ -122,6 +127,11 @@ describe('PathUtil', (): void => {
     it('leaves the query string untouched.', (): void => {
       expect(encodeUriPathComponents('/a%20path&/name?abc=def&xyz')).toBe('/a%2520path%26/name?abc=def&xyz');
     });
+
+    it('does not double-encode url encoded path separator characters.', (): void => {
+      expect(encodeUriPathComponents('/a%20path&/c1/c2/t1%2F')).toBe('/a%2520path%26/c1/c2/t1%2F');
+      expect(encodeUriPathComponents('/a%20path&/c1/c2/t1%5C')).toBe('/a%2520path%26/c1/c2/t1%5C');
+    });
   });
 
   describe('#isContainerPath', (): void => {
diff --git a/test/util/Util.ts b/test/util/Util.ts
index e96f2c1af..c6563b3ef 100644
--- a/test/util/Util.ts
+++ b/test/util/Util.ts
@@ -8,6 +8,7 @@ const portNames = [
   'ContentNegotiation',
   'DynamicPods',
   'ExpiringDataCleanup',
+  'FileBackendEncodedSlashHandling',
   'GlobalQuota',
   'Identity',
   'LpdHandlerWithAuth',